slurry packing disposable polyethylene chromatography columns Search Results


hplc slurry packing system  (Chrom Tech)
86
Chrom Tech hplc slurry packing system
Hplc Slurry Packing System, supplied by Chrom Tech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/hplc+packing+slurry+system/pm31079009-56-1-6
Average 86 stars, based on 1 article reviews
hplc slurry packing system - by Bioz Stars, 2026-09
86/100 stars
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strep tactin super flow high capacity slurry  (IBA Lifesciences)
96
IBA Lifesciences strep tactin super flow high capacity slurry
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Strep Tactin Super Flow High Capacity Slurry, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/Strep-Tactin/pmc08874384-45-9-13
Average 96 stars, based on 1 article reviews
strep tactin super flow high capacity slurry - by Bioz Stars, 2026-09
96/100 stars
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chromatography media  (Omnifit Inc)
90
Omnifit Inc chromatography media
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Chromatography Media, supplied by Omnifit Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/chromatography+media/us11229896-355-8-21
Average 90 stars, based on 1 article reviews
chromatography media - by Bioz Stars, 2026-09
90/100 stars
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gravity flow poly prep chromatography columns  (Bio-Rad)
99
Bio-Rad gravity flow poly prep chromatography columns
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Gravity Flow Poly Prep Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/Columns+For+Chromatography+Poly-Prep/pmc06125173-200-7-12
Average 99 stars, based on 1 article reviews
gravity flow poly prep chromatography columns - by Bioz Stars, 2026-09
99/100 stars
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econo pac chromatography column  (Bio-Rad)
96
Bio-Rad econo pac chromatography column
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Econo Pac Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/Econo-Pac+Chromatography+Columns/pmc04986992-139-16-19
Average 96 stars, based on 1 article reviews
econo pac chromatography column - by Bioz Stars, 2026-09
96/100 stars
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reprosil-pur c18-aq 3- m reverse phase resin  (Dr Maisch HPLC)
90
Dr Maisch HPLC reprosil-pur c18-aq 3- m reverse phase resin
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Reprosil Pur C18 Aq 3 M Reverse Phase Resin, supplied by Dr Maisch HPLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/reprosil+pur+c18+aq+resin/10__1074_slash_mcp__m700354___mcp200-97-32-39
Average 90 stars, based on 1 article reviews
reprosil-pur c18-aq 3- m reverse phase resin - by Bioz Stars, 2026-09
90/100 stars
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liquid chromatography-tandem mass chromatograpy  (Midwest Bio Services LLC)
90
Midwest Bio Services LLC liquid chromatography-tandem mass chromatograpy
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Liquid Chromatography Tandem Mass Chromatograpy, supplied by Midwest Bio Services LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/liquid+chromatography+tandem+mass+chromatograpy/pmc02999476-226-12-16
Average 90 stars, based on 1 article reviews
liquid chromatography-tandem mass chromatograpy - by Bioz Stars, 2026-09
90/100 stars
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acetonitrile chromatographic grade  (Merck KGaA)
90
Merck KGaA acetonitrile chromatographic grade
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Acetonitrile Chromatographic Grade, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/chromatographic+grade+acetonitrile/10__1590_slash_s0102___695x2012005000143-37-4-10
Average 90 stars, based on 1 article reviews
acetonitrile chromatographic grade - by Bioz Stars, 2026-09
90/100 stars
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biotage flash chromatographer  (BIOTAGE)
90
BIOTAGE biotage flash chromatographer
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Biotage Flash Chromatographer, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/biotage+isolera+one/pmc11253158__SC___015___D4SC02767A___s001-704-8-10
Average 90 stars, based on 1 article reviews
biotage flash chromatographer - by Bioz Stars, 2026-09
90/100 stars
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ion chromatograph  (Metrohm AG)
90
Metrohm AG ion chromatograph
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Ion Chromatograph, supplied by Metrohm AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/ion+chromatography/pmc10707126-84-0-5
Average 90 stars, based on 1 article reviews
ion chromatograph - by Bioz Stars, 2026-09
90/100 stars
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compact ion chromatograph (ic) 861 chromatography instrument  (Metrohm AG)
90
Metrohm AG compact ion chromatograph (ic) 861 chromatography instrument
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Compact Ion Chromatograph (Ic) 861 Chromatography Instrument, supplied by Metrohm AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/compact+ion+chromatograph/pmc04543969-43-22-21
Average 90 stars, based on 1 article reviews
compact ion chromatograph (ic) 861 chromatography instrument - by Bioz Stars, 2026-09
90/100 stars
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radial chromatography  (Merck & Co)
86
Merck & Co radial chromatography
Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells <t>and</t> <t>purified</t> via affinity chromatography using <t>Strep-Tactin</t> resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.
Radial Chromatography, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slurry+packing+disposable+polyethylene+chromatography+columns/chromatography+radial/pmc12481153-47-5-14
Average 86 stars, based on 1 article reviews
radial chromatography - by Bioz Stars, 2026-09
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Image Search Results


Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells and purified via affinity chromatography using Strep-Tactin resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.

Journal: Vaccines

Article Title: Immunization with GP1 but Not Core-like Particles Displaying Isolated Receptor-Binding Epitopes Elicits Virus-Neutralizing Antibodies against Junín Virus

doi: 10.3390/vaccines10020173

Figure Lengend Snippet: Preparation of antigens for immunization. ( A ) Purification of recombinant Junín virus (JUNV) GP1. JUNV GP1 containing a C-terminal Strep tag (GP1-Strep) was transiently expressed in HEK-293 cells and purified via affinity chromatography using Strep-Tactin resin. The eluted fractions were analyzed by Coomassie staining (left panel). Alternatively, JUNV GP1 containing a C-terminal hexahistidine (GP1-His) tag was expressed in Freestyle 293-F cells and purified via affinity chromatography using HisTrap columns. The resulting protein fraction was also analyzed by Coomassie staining (right panel). ( B ) Hepatitis B virus core-like particles (HBV-CLPs) carrying JUNV GP1 peptide loops. HBV-CLPs containing the indicated antigens (i.e., Loop 3, Loop 7, Loop 10, or a control FLAG tag) were produced in E. coli and isolated from the soluble fraction of lysates via Strep tag-mediated affinity purification. Eluates were analyzed by Coomassie staining (upper panel) and Western blot with an anti-Strep antibody (1:30,000; StrepMAB Classic-HRP; IBA Lifesciences, Göttingen, Germany; lower panel). The position of the HBV core protein is indicated. ( C ) Electron microscopic analysis of HBV-CLPs. The purified HBV-CLPs generated in ( B ) were further analyzed by electron microscopy with negative staining. The scale bar shown represents 80 nm.

Article Snippet: The protein was then purified by affinity chromatography using Strep-Tactin Super-flow high-capacity slurry (IBA Lifesciences, Göttingen, Germany) following the manufacturer’s instructions.

Techniques: Purification, Recombinant, Strep-tag, Affinity Chromatography, Staining, FLAG-tag, Produced, Isolation, Affinity Purification, Western Blot, Generated, Electron Microscopy, Negative Staining